Pressure steam sterilization speed biological indicator
TRYC brand pressure steam sterilization effect monitoring speed biological indicator instruction manualI. Product Description
This product uses TRYC brand extreme speed biological culture reader to detect the activity of α -glucosidase within 0.5h, and to monitor the survival of Bacillus thermophilus spores, so as to judge whether the pressure steam sterilization is qualified.
Second, the scope of use
It is suitable for monitoring the sterilization effect of pressure steam.
Third, the method of use
1. Record the necessary items of sterilization management (such as sterilization date, operator, etc.) in the blank space of the label on the surface of the high-speed biological indicator for monitoring the sterilization effect of pressure steam.
2. Place the rapid biological indicator for monitoring the sterilization effect of pressure steam in the center of the standard test package, and then place the standard test package above the exhaust port of the sterilizer or at the position difficult to sterilize in the sterilizer suggested by the manufacturer to run the sterilization program.
3. After sterilization, take out the biological indicator from the standard test package, and after confirming that the chemical indicator ink on the label changes color, naturally leave it for more than 15min and cool it to room temperature. If the chemical indication ink on the label has not changed, please check the sterilization process.
4. Press the upper cover of the biological indicator tightly, and pinch the ampoule bottle inside the cooled biological indicator with a clip or in a specific hole in the extreme speed biological incubator. Shake to make the bacterial slices fully contact the liquid culture medium, confirm that the bacterial slices are immersed in the culture medium, and cultivate them in TRYC brand extreme speed biological culture reader.
5. Take another unsterilized biological indicator with the same batch number, and culture it as a positive control under the same conditions after the ampoule is broken. The purpose is to detect the correct culture conditions, the activity of indicators and the function of the extreme speed biological incubator.
6. Open the lid of the extreme speed biological incubator, put the extreme speed biological indicator for monitoring the sterilization effect of pressure steam into the culture hole in the incubator, close the lid of the incubator, and culture the positive control and the sterilized extreme speed biological indicator for 0.5 hours, waiting for the red or green indicator light to light up and send out the result signal. If you want to observe the color change of biological indicators, please culture for 24 hours.
7. After confirming the sterilization effect, please remove the label on the surface of the biological indicator and stick it in the record book.
Determination of fluorescence detection results:
1. For the indicator after sterilization, a fluorescent positive (red light or+) indicates that sterilization is unqualified.
2. For the indicator after sterilization, negative fluorescence (green light or-) indicates that sterilization is qualified.
3, the positive control must get a fluorescent positive result, and the biological monitoring result is effective.
Fourth, matters needing attention
1. Before using this product, please confirm the integrity of the product and use it within the validity period.
2, stored at 15℃-25℃ and dry environment (relative humidity 35%-60%), away from light (including sunlight, fluorescent lamps and ultraviolet disinfection lights).
3. Biological indicators that have been judged to have failed sterilization, expired and used in positive control tests should be discarded after sterilization.
4. This product cannot be used for biological monitoring of dry heat, ethylene oxide and other low-temperature sterilization processes.
5. Validity: 24 months. See the product packaging for the production date, batch number and specifications.
Manufacturer: Anhui Tianrun Medical New Materials Co., Ltd.
Production address: No.29 Chaoyang Road, Yixiu District, Anqing City, Anhui Province
Production license: Wan Wei Xiao Zheng Zi [2023]No. H0047
Tel: 0556-5877077 Postal code: 246500
Fax: 0556-5877079 Website: www.ahtrbz.com.
1. Record the necessary items of sterilization management (such as sterilization date, operator, etc.) in the blank space of the label on the surface of the high-speed biological indicator for monitoring the sterilization effect of pressure steam.
2. Place the rapid biological indicator for monitoring the sterilization effect of pressure steam in the center of the standard test package, and then place the standard test package above the exhaust port of the sterilizer or at the position difficult to sterilize in the sterilizer suggested by the manufacturer to run the sterilization program.
3. After sterilization, take out the biological indicator from the standard test package, and after confirming that the chemical indicator ink on the label changes color, naturally leave it for more than 15min and cool it to room temperature. If the chemical indication ink on the label has not changed, please check the sterilization process.
4. Press the upper cover of the biological indicator tightly, and pinch the ampoule bottle inside the cooled biological indicator with a clip or in a specific hole in the extreme speed biological incubator. Shake to make the bacterial slices fully contact the liquid culture medium, confirm that the bacterial slices are immersed in the culture medium, and cultivate them in TRYC brand extreme speed biological culture reader.
5. Take another unsterilized biological indicator with the same batch number, and culture it as a positive control under the same conditions after the ampoule is broken. The purpose is to detect the correct culture conditions, the activity of indicators and the function of the extreme speed biological incubator.
6. Open the lid of the extreme speed biological incubator, put the extreme speed biological indicator for monitoring the sterilization effect of pressure steam into the culture hole in the incubator, close the lid of the incubator, and culture the positive control and the sterilized extreme speed biological indicator for 0.5 hours, waiting for the red or green indicator light to light up and send out the result signal. If you want to observe the color change of biological indicators, please culture for 24 hours.
7. After confirming the sterilization effect, please remove the label on the surface of the biological indicator and stick it in the record book.
Determination of fluorescence detection results:
1. For the indicator after sterilization, a fluorescent positive (red light or+) indicates that sterilization is unqualified.
2. For the indicator after sterilization, negative fluorescence (green light or-) indicates that sterilization is qualified.
3, the positive control must get a fluorescent positive result, and the biological monitoring result is effective.
Fourth, matters needing attention
1. Before using this product, please confirm the integrity of the product and use it within the validity period.
2, stored at 15℃-25℃ and dry environment (relative humidity 35%-60%), away from light (including sunlight, fluorescent lamps and ultraviolet disinfection lights).
3. Biological indicators that have been judged to have failed sterilization, expired and used in positive control tests should be discarded after sterilization.
4. This product cannot be used for biological monitoring of dry heat, ethylene oxide and other low-temperature sterilization processes.
5. Validity: 24 months. See the product packaging for the production date, batch number and specifications.
Manufacturer: Anhui Tianrun Medical New Materials Co., Ltd.
Production address: No.29 Chaoyang Road, Yixiu District, Anqing City, Anhui Province
Production license: Wan Wei Xiao Zheng Zi [2023]No. H0047
Tel: 0556-5877077 Postal code: 246500
Fax: 0556-5877079 Website: www.ahtrbz.com.
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